We can customize various antibody libraries with a capacity of 108 or more for our clients. Including human, camels, mice, rabbits and other species.
After years of practice and experience, Gegen can generate high capacity human natural antibody libraries by using optimized technology including cloning, enzyme digestion , connection, and electrical transformation. Gegen has constructed human Fab antibody library, ScFV antibody library and Semi-synthetic antibody library with a size of 2 x 1011, 5 x1011 and 4 x 1012贵州福彩网 respectively.
Nanobodies: Because of their small size, high affinity, high safe protein materials, access to enzymatic active sites to provide potent enzyme inhibition, Nanobodies can be ideal alternative agents to small molecular chemicals.
贵州福彩网Hybridomas are cells that have been engineered to produce a desired antibody in large amounts. B cells are removed from the spleen of an animal that has been challenged with the relevant antigen fused with immortal B cell cancer cells, a myeloma, to produce a hybrid cell line called a hybridoma, which has both the antibody-producing ability of the B-cell and the exaggerated longevity and reproductivity of the myeloma.
贵州福彩网After humanization, high affinity mouse monoclonal antibody can not only ensure the same affinity, but also reduce the immunogenicity.
Chimeric Antigen Receptor T-cell therapy (CAR-T) therapy is one of the most effective Immunotherapy methods for malignant tumors. Chimeric antigen receptor (CAR) is the core parts of the CAR – T, which is an engineered T-cell receptor (TCR) construct by fusion tumor associate antigen-specific ScFv antibody with an extracellular hinge area, a transmembrane domain (TMD) and an intracellular T-cell signaling domain.
A specific antigen gene is digested by endonuclease. The gene fragment cocktails with different sizes are inserted into phage display vector. The constructed antigen phage display library is screened by specific antibody. Antibodies against specific epitopes can obtained.
Small Molecular ScFv Antibodies—The alternatives of intact antibodies in the antibody family.
ScFv antibodies contain only antibody variable regions with a molecular weight about 27 kDa and are as specificity and affinity as intact antibodies. In addition, they can be easily produced by recombinant expression. ScFv antibodies are excellent alternatives for antibody drug development.
VHH Antibodies—Novel structural antibodies further expanded antibody application areas in the antibody family.
VHH antibodies contain only the variable regions of heavy chain of camelid antibodies and have very small sizes of around 15 kDa. VHH antibodies are easy for expression and conjugation in E. coli. The unique features of small size and high affinity of VHH antibodies enable applications ranging from their use as biochemical tools to therapeutic agents. VHH antibodies are ideal alternative therapeutic regents for small molecular chemicals.
Bispecific ScFv Antibodies—Next generation antibody constructs for antitumor drugs.
Bispecific ScFv Antibodies are engineered antibodies capable of simultaneously binding and neutralizing two different antigens. They can serve as mediators in redirecting immune effectors and can pass through the blood-brain barrier. The anti-tumor effect is more obvious than that of traditional antibody drugs.
Chimeric Antigen Receptor T-Cell(CAR-T) Immunotherapy-One of the most promising treatments against cancers.
Bispecific ScFv antibodies are indispensable components of CAR-T. Gegen provides bispecific ScFv antibody genes for CAR-T treatment.
贵州福彩网 With the professional technology and extensive experience, Gegen provides its customers with the highest quality products and services in a cost-effective way.