Tailor-made phage antibody library
We can customize various antibody libraries with a capacity of 108贵州福彩网 or more for our clients. Including human, camels, mice, rabbits and other species.
1. Total RNA is extracted from peripheral blood mononuclear cells (PBMC) and reverse transcribed into cDNA
贵州福彩网2. The whole antibody light chain and heavy chain genes are amplified by PCR
贵州福彩网3. Antibody VL and VH genes are assembled and cloned in phage expression vectors, and then transformed into E Coli.
4. Bacteria are cultured with selective antibiotic, and auxiliary phages are added.
5. Phage antibody library are harvesed(Fab antibody library, ScFV antibody library, nanobody library)