Phage display technology platform
Recombinant antibodies have emerged as valuable and practical means for research, diagnosis, and therapeutic proteins. Phage Display is a technique of reference for in vitro antibody production. The antibody sequences are cloned in fusion with the gIIIp envelop protein of the M13 phage in expression vector and displayed as single-chain variable fragment or Fab-fragment antibodies on the surface of filamentous bacteriophage. Specific antibodies are obtained by several rounds of selection. Selected antibodies recognizing specific antigens are transformed in bacteria for amplification. Compared to the classical hybridoma system, phage surface display technology could obtain human antibodies directly.
Gegen can provide tailor-made human natural antibody libraries and human semi-synthetic antibody libraries. The natural phage antibody libraries are made of cDNA fragments encoding heavy and light variable regions of antibodies amplified from human B lymphocyte and expressed on the surface of phage. The semi-synthetic phage antibody libraries are made by insertion random nucleotide sequences into the CDR3 region.
Gegen has a variety of phage display systems that can display 7 to 20 short peptides as well as proteins within 50KD .
Gegen has decades experience in the generation of large capacity antibody libraries. The clone repertories of libraries are above 108.