Nano antibody preparation platform
贵州福彩网Camelid antibodies have all the characteristics we expect from mammalian immune systems. However, except conventional IgG1 antibodies, they also include IgG2 and IgG3, a group of smaller antibodies containing only heavy chain dimers without the light chains altogether. The molecular weight of heavy chain antibodies is about half of the conventional antibody. Because of their small size, heavy chain antibodies can even enter tissue cells to take functions. In addition to camelids, sharks also have heavy chain antibodies. The incomplete heavy chain antibodies are thought to be a conservative remnant in the evolutionary process which provide these species extra adaptive ability to environment.
贵州福彩网Since the discovery of camel heavy chain antibodies, single domain antibodies were generated from camelid animals through genetic engineering methods. The so-called VHH or nanobody, corresponds to the variable region of a heavy chain of a camelid antibody and has a very small size of around 15 kDa.
They obtained variable region of camel heavy chain antibody, its molecular weight is 15 kd, called nanobodies. VHHs are high affinity, strong hydrophilicity, acid and alkali resistance and thermostable. This type of affinity molecules are easy to express in E. coli. With nanobody patent of Belgian Ablynx expiration in 2014, and its first nanobody drug approved in 2018, the company is bought by Sanofi with <.8 billion. Nanobodies will become the next generation lead antibodies for diagnostic and therapeutic drugs.
贵州福彩网Nanobody preparation procedure